Hwang S R, Kohn A B, Hook V Y
Department of Medicine, UCSD Medical Center 92103-8227.
Biochem Biophys Res Commun. 1995 Feb 15;207(2):701-7. doi: 10.1006/bbrc.1995.1244.
Sulfate conjugation by sulfotransferases is important for the metabolism of drugs, xenobiotics, monoamines, and neuropeptides (1-3). In this study, molecular cloning resulted in the isolation of a 1.2-kb human brain hippocampal cDNA encoding a member of the phenol sulfotransferase (PST) family of enzymes. The hippocampal phenol sulfotransferase (H-PST) cDNA possesses differences in its deduced primary sequence compared to human liver monoamine (M form) (4,5) and phenol (P form) (6,7) isoforms of PST. The hippocampal PST contrasts with previous studies that identified two human brain PST cDNAs whose coding sequences are identical to the human liver M and P forms of PST. Importantly, this study provides evidence for a human hippocampal form of PST.
硫酸转移酶介导的硫酸化结合反应对于药物、外源性物质、单胺类物质和神经肽的代谢具有重要意义(1 - 3)。在本研究中，通过分子克隆从人脑海马区分离出一个1.2 kb的cDNA，其编码酚硫酸转移酶(PST)家族的一个成员。与人类肝脏单胺(M型)(4,5)和酚(P型)(6,7)亚型的PST相比，脑海马酚硫酸转移酶(H - PST) cDNA的推导一级序列存在差异。海马PST与之前的研究不同，之前的研究鉴定出两个人脑PST cDNA，其编码序列与人类肝脏PST的M型和P型相同。重要的是，本研究为人类海马型PST提供了证据。
