Zhu X, Veronese M E, Bernard C C, Sansom L N, McManus M E
Department of Physiology and Pharmacology, University of Queensland, Australia.
Biochem Biophys Res Commun. 1993 Aug 31;195(1):120-7. doi: 10.1006/bbrc.1993.2018.
A 1,179 bp and a 1,424 bp full-length aryl sulfotransferase cDNAs were isolated from a human brain cDNA library. Their coding domains are 93% identical, each encoding a cytosolic protein of 295 amino acids. Their deduced amino acid sequences of these cDNAs are also 93% identical. The 1179 bp brain cDNA has an identical coding domain to a previously reported human liver aryl sulfotransferase cDNA but it has a different 5' noncoding sequence. Northern blot analysis using a probe specific for the 1,424 bp cDNA identified a 1500 bp band in mRNA of human liver, colon, kidney and lung. In a human hepatocellular carcinoma the same band plus an extra larger band was also recognised. An intron of the gene encoding the 1424 bp cDNA was also identified.
从人脑cDNA文库中分离出了一个1179 bp和一个1424 bp的全长芳基硫酸转移酶cDNA。它们的编码结构域有93%的同一性，各自编码一个由295个氨基酸组成的胞质蛋白。这些cDNA推导的氨基酸序列也有93%的同一性。1179 bp的脑cDNA与先前报道的人肝脏芳基硫酸转移酶cDNA有相同的编码结构域，但有不同的5'非编码序列。使用针对1424 bp cDNA的特异性探针进行的Northern印迹分析，在人肝脏、结肠、肾脏和肺的mRNA中鉴定出一条1500 bp的条带。在人肝细胞癌中也识别出了相同的条带以及一条额外的更大条带。还鉴定出了编码1424 bp cDNA的基因的一个内含子。
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