在小鼠乳腺中检测到促性腺激素释放激素（GnRH）的信使核糖核酸，但未检测到GnRH受体的信使核糖核酸。

Detection of messenger RNA for gonadotropin-releasing hormone (GnRH) but not for GnRH receptors in mouse mammary glands.

作者信息

Ikeda M, Taga M, Sakakibara H, Minaguchi H, Vonderhaar B K

机构信息

Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1995 Feb 15;207(2):800-6. doi: 10.1006/bbrc.1995.1257.

DOI:10.1006/bbrc.1995.1257

PMID:7864875

Abstract

While gonadotropin-releasing hormone (GnRH), GnRH-like, or GnRH receptor (GnRH-R) have been reported to exist in several tissues other than brain or anterior pituitary, there is no report concerning GnRH or GnRH-R gene expression in the normal mammary gland. In order to define the production of GnRH as well as GnRH-R in the mammary gland at the molecular level we examined their gene expression in various functional stages of the mouse mammary gland using the reverse transcriptase-polymerase chain reaction (RT-PCR). GnRH mRNA transcripts were found in mouse mammary glands of mid-pregnant, lactating, and 3, 6, 9 days post-lactational mice, whereas GnRH-R mRNA transcripts were not detected in mammary glands of any functional stage. These results suggest a possible biological role of GnRH in mammary gland.

摘要

虽然据报道促性腺激素释放激素（GnRH）、GnRH类似物或GnRH受体（GnRH-R）存在于脑或垂体前叶以外的多种组织中，但尚无关于正常乳腺中GnRH或GnRH-R基因表达的报道。为了在分子水平上确定乳腺中GnRH以及GnRH-R的产生情况，我们使用逆转录聚合酶链反应（RT-PCR）检测了它们在小鼠乳腺不同功能阶段的基因表达。在妊娠中期、哺乳期以及哺乳后3、6、9天的小鼠乳腺中发现了GnRH mRNA转录本，而在任何功能阶段的乳腺中均未检测到GnRH-R mRNA转录本。这些结果提示GnRH在乳腺中可能具有生物学作用。