Huang S Z, Ren Z R, Chen M J, Xu H P, Zeng Y T, Rodgers G P, Zeng F Y, Schechter A N
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, PRC.
Sci China B. 1994 Nov;37(11):1350-9.
A newly developed method of RT-PCR/competitive PCR for measuring the relative and absolute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study the alterations of globin gene expressions in the patients with beta-thalassemia pre- and post-hydroxyurea (HU) treatment. It was found for the first time that HU had the effect of enhancing beta-globin gene expression in some patients. Two cases with beta-thalassemia who were subjected to HU treatment for over two years showed a marked increase in beta-globin mRNA level and beta-globin chain synthesis, resulting in more effective erythropoiesis and the alleviation of clinical symptoms.
一种新开发的用于测量珠蛋白mRNA相对和绝对含量的逆转录聚合酶链反应/竞争性聚合酶链反应方法以及微球蛋白链生物合成测定法，已被用于研究β地中海贫血患者在羟基脲（HU）治疗前后珠蛋白基因表达的变化。首次发现HU对某些患者有增强β珠蛋白基因表达的作用。两名接受HU治疗超过两年的β地中海贫血患者，其β珠蛋白mRNA水平和β珠蛋白链合成显著增加，从而使红细胞生成更有效，并缓解了临床症状。
