Kim S Y, Lee K J, Hong S C, Han P S, Lee J J, Cho H J, Kim A K, Kim J O, Lee M S
Department of Internal Medicine, Chungnam National University, Taejon, Korea.
Korean J Intern Med. 1994 Jul;9(2):55-60. doi: 10.3904/kjim.1994.9.2.55.
Since conventional cytogenetic analysis for bronchogenic carcinogenesis is limited by the difficulty to get enough number of high quality metaphase spreads, the development of new method to overcome above problems is strongly needed. Therefore, the introduction of non-radioactive in situ hybridization (ISH) with pericentromeric chromosome probes gave us the way to investigate the genetic events during carcinogenic process. We applied this method on lung cancer tissue to validate the possibility of this method for general usage and to analyze numerical chromosome aberration status and their clinical correlations.
A set of satellite DNA probes specific for chromosome 3, 7, 9, 11, and 17 was hybridized directly to paraffin-embedded tissue section of 30 non-small cell lung cancers. Mean chromosome index of each chromosome and frequency of polysomy for each chromosome were calculated.
Mean chromosome indices for chromosome 3, 7, 9, 11, and 17 were 1.10, 1.13, 1.17, 1.12, and 1.17, respectively. Polysomy for a set of chromosomes was detected in all 30 cases except 4 cases which showed hypoploidy only for chromosome 3 or 7 in 2 cases and diploidy only for chromosome 3 or 11 in 2 cases. Among the set of chromosomes, mean chromosome index and polysomy frequency for chromosome 9 & 17 were significantly higher than that for others. Mean chromosome index or polysomy pattern for each chromosome was not much different among cell types or clinical stages.
Our results show that chromosome ISH can be used to screen for numerical chromosome aberrations on paraffin tissue sections and further studies for ISH analysis with different probes on same tumor area or double-target ISH in large scale are needed to confirm above results and to elucidate the specific meanings.
由于用于支气管癌发生的传统细胞遗传学分析受限于难以获得足够数量的高质量中期分裂相，因此迫切需要开发新方法来克服上述问题。因此，采用着丝粒周围染色体探针的非放射性原位杂交（ISH）为我们研究致癌过程中的遗传事件提供了途径。我们将此方法应用于肺癌组织，以验证该方法广泛应用的可能性，并分析染色体数目畸变状况及其临床相关性。
将一组针对3号、7号、9号、11号和17号染色体的卫星DNA探针直接与30例非小细胞肺癌的石蜡包埋组织切片杂交。计算每条染色体的平均染色体指数和每条染色体的多体频率。
3号、7号、9号、11号和17号染色体的平均染色体指数分别为1.10、1.13、1.17、1.12和1.17。在所有30例病例中均检测到一组染色体的多体现象，只有4例除外，其中2例仅显示3号或7号染色体的亚二倍体，2例仅显示3号或11号染色体的二倍体。在这组染色体中，9号和17号染色体的平均染色体指数和多体频率显著高于其他染色体。各染色体的平均染色体指数或多体模式在细胞类型或临床分期之间差异不大。
我们的结果表明，染色体ISH可用于筛查石蜡组织切片上的染色体数目畸变，需要进一步对同一肿瘤区域使用不同探针进行ISH分析或大规模双靶点ISH研究，以证实上述结果并阐明其具体意义。
