Uchida T, Nakamura T, Nakai Y, Ogimoto K
Department of Animal Microbiology and Parasitology, Tohoku University, Sendai, Japan.
J Vet Med Sci. 1994 Oct;56(5):1021-3. doi: 10.1292/jvms.56.1021.
Cross reactivity of serum antibodies from chicken immunized with E. tenella, E. necatrix and E. acervulina was observed. The enzyme linked immunosorbent assay (ELISA) using soluble antigen prepared from oocysts of each species showed the cross reactivity among chicken serum immunized with the three species of protozoa, especially between E. tenella and E. necatrix. In immunoblot analysis with E. tenella oocyst antigen, 7 major bands were detected by homologous E. tenella antiserum and 10 and 6 bands were reacted by E. necatrix and E. acervulina antiserum respectively. In these antigens, the band of 33 kDa was regarded as cross reactive agent between E. tenella and E. necatrix because of high reactivity to both of the antiserum. In addition, the 130 kDa antigen that reacted with every antiserum was regarded as common antigen among these three species.
观察了用柔嫩艾美耳球虫、毒害艾美耳球虫和堆型艾美耳球虫免疫的鸡血清抗体的交叉反应性。使用从每种球虫卵囊制备的可溶性抗原进行的酶联免疫吸附测定（ELISA）显示，用这三种原生动物免疫的鸡血清之间存在交叉反应性，尤其是在柔嫩艾美耳球虫和毒害艾美耳球虫之间。在用柔嫩艾美耳球虫卵囊抗原进行的免疫印迹分析中，同源的柔嫩艾美耳球虫抗血清检测到7条主要条带，毒害艾美耳球虫和堆型艾美耳球虫抗血清分别有10条和6条条带发生反应。在这些抗原中，33 kDa的条带由于对两种抗血清都有高反应性，被认为是柔嫩艾美耳球虫和毒害艾美耳球虫之间的交叉反应剂。此外，与每种抗血清都发生反应的130 kDa抗原被认为是这三个物种之间的共同抗原。
