Scrutton M C, Ross-Murphy S B, Bennett G M, Stirling Y, Meade T W
Division of Life Sciences, King's College, London.
Blood Coagul Fibrinolysis. 1994 Oct;5(5):719-23. doi: 10.1097/00001721-199410000-00007.
This study examined the rheological properties of fibrin gels formed by adding thrombin to plasma samples from 99 subjects with fibrinogen concentrations ranging from 1.45 to 4.14 g/l. A highly significant (r = 0.757; P < 0.001) inverse correlation was observed between plasma fibrinogen concentration and the extent of clot deformability as estimated from the final value of the storage modulus (G') of the fibrin gel when obtained by rheological analysis. A similarly significant correlation (r = 0.844; P < 0.001) was obtained using samples from 47 subjects in which fibrin cross-linking was blocked by addition of 0.1 mM iodoacetamide to inactivate factor XIIIa. The characteristics of the relationship between G' and fibrinogen concentration in the plasma samples was comparable with that observed when the fibrin gel was formed by adding thrombin to purified fibrinogen. These results suggest that the increased risk of myocardial infarction associated with an elevated plasma fibrinogen concentration may, in part, be explained on the basis of a decreased deformability of the fibrin clot formed.
本研究检测了向99名纤维蛋白原浓度在1.45至4.14 g/l之间的受试者的血浆样本中添加凝血酶后形成的纤维蛋白凝胶的流变学特性。通过流变学分析获得纤维蛋白凝胶的储能模量（G'）的最终值来估计，血浆纤维蛋白原浓度与血凝块变形程度之间存在高度显著的负相关（r = 0.757；P < 0.001）。使用47名受试者的样本也获得了类似显著的相关性（r = 0.844；P < 0.001），在这些样本中，通过添加0.1 mM碘乙酰胺使因子XIIIa失活来阻断纤维蛋白交联。血浆样本中G'与纤维蛋白原浓度之间关系的特征与向纯化纤维蛋白原中添加凝血酶形成纤维蛋白凝胶时观察到的特征相当。这些结果表明，血浆纤维蛋白原浓度升高与心肌梗死风险增加之间的关联，部分可能是由于所形成的纤维蛋白凝块变形性降低所致。
