Chen J, Zhang Y, Wu Y
Beijing Institute of Radiation Medicine.
Chin Med Sci J. 1994 Sep;9(3):143-6.
O6-methylguanine-DNA methyltransferase (MGMT) gene expression in 6 Mer+ (HeLa S3, SMMC-7721, SGC-7901, B-239, AGZY83-a, and Cc801) and 2 Mer- (SHG-44, and HeLa MR) human tumor cell lines was examined. Southern blot analysis revealed no deletion, amplification, or rearrangement of the MGMT gene in these cell lines. However, approximately 1.0 kb transcripts were detected in the 6 Mer+ cell lines but not in the 2 Mer- cell lines by Northern blot analysis. Furthermore, a rough correlation between MGMT activity and mRNA level in these cell lines was observed. These results suggest that transcriptional regulation of the MGMT gene is the molecular basis of the absence of MGMT activity in Mer- cell lines.
检测了6种Mer+（HeLa S3、SMMC - 7721、SGC - 7901、B - 239、AGZY83 - a和Cc801）和2种Mer-（SHG - 44和HeLa MR）人肿瘤细胞系中O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）基因的表达。Southern印迹分析显示这些细胞系中MGMT基因无缺失、扩增或重排。然而，通过Northern印迹分析在6种Mer+细胞系中检测到约1.0 kb的转录本，而在2种Mer-细胞系中未检测到。此外，观察到这些细胞系中MGMT活性与mRNA水平之间存在大致的相关性。这些结果表明，MGMT基因的转录调控是Mer-细胞系中缺乏MGMT活性的分子基础。
