Liu A, Zhu P, Li X, Zhang L, Chi F, Wu Z, Song Y, Zhang Y
Department of Histology and Embryology, Harbin Medical University.
Chin Med Sci J. 1994 Sep;9(3):157-61.
Reciprocal interactions between hemopoietic stromal cells and immature hemopoietic cells in human spleens obtained from 20 fetuses of 10-28 weeks gestation were observed by transmission electron microscopy and scanning electron microscopy. The close association of stromal cells with immature hemopoietic cells was confirmed under the electron microscope and a presumptive HIM (Hemopoietic inductive microenvironment) was visualized. In regions of immature hemopoietic cell-reticular cell, endothelial cell, macrophage and interdigitating cell contact, some communicating structures were found between the plasma membranes of adjacent cells; moreover, the cytoplasm of these four stromal cells were full of various kinds of organelles. These results suggest that reticular cells, endothelial cells, macrophages and interdigitating cells are component parts of the HIM of human fetal spleen and that these cells have a nurturing function in relation to hemopoietic cells.
通过透射电子显微镜和扫描电子显微镜观察了从妊娠10 - 28周的20例胎儿获取的人脾脏中造血基质细胞与未成熟造血细胞之间的相互作用。在电子显微镜下证实了基质细胞与未成熟造血细胞的紧密联系，并观察到了推测的造血诱导微环境（HIM）。在未成熟造血细胞与网状细胞、内皮细胞、巨噬细胞和指状突细胞接触的区域，相邻细胞的质膜之间发现了一些通讯结构；此外，这四种基质细胞的细胞质中充满了各种细胞器。这些结果表明，网状细胞、内皮细胞、巨噬细胞和指状突细胞是人类胎儿脾脏造血诱导微环境的组成部分，并且这些细胞对造血细胞具有滋养功能。
