Petithory J C, Brumpt L C, Poujade F
Département de biologie médicale E. Brumpt, Centre Hospitalier, Gonesse.
Bull Soc Pathol Exot. 1994;87(4):231-7.
On the basis of epidemiologic and clinical data in humans, and of experimental studies in kittens, E. Brumpt in 1925 showed that Entamoeba histolytica producing quadrinucleate cysts was a complex of 2 species. He differentiated a pathogenic and invasive form, from a non pathogenic and non invasive one that he called Entamoeba dispar. His explanation gained little support until recently, when Sargeaunt reported a study of isoenzyme typing and allow then the classification of amoebae resembling E. histolytica in pathogenic and non pathogenic species. Monoclonal antibodies and PCR have recently been used and have shown to react specifically with the two forms of amoebae. Genetic evidence has also demonstrated that the pathogenic and non pathogenic amoebae represent different species.
基于人类的流行病学和临床数据以及对小猫的实验研究，E. 布伦普特在1925年指出，产生四核包囊的溶组织内阿米巴是两个物种的复合体。他区分出一种致病且具有侵袭性的类型，以及一种非致病且无侵袭性的类型，后者他称之为迪斯帕内阿米巴。直到最近他的解释才得到了一些支持，当时萨金特报告了一项关于同工酶分型的研究，进而得以将类似溶组织内阿米巴的阿米巴分为致病和非致病物种。单克隆抗体和聚合酶链反应（PCR）最近已被使用，并显示出能与这两种阿米巴形式发生特异性反应。遗传学证据也已证明，致病和非致病阿米巴代表不同的物种。
