Seenaiah B, Ellingson J S
Department of Pathology and Cell Biology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.
J Chromatogr B Biomed Appl. 1994 Oct 14;660(2):380-5. doi: 10.1016/0378-4347(94)00308-4.
A reversed-phase HPLC method to monitor the incorporation of radiolabeled precursors into the polar group of individual polyunsaturated molecular species of phosphatidylserine (PS) is presented. PS labeled in the polar group was decarboxylated and subsequently converted to trinitrophenyl-phosphatidylethanolamine (Tnp-PE), which was separated into its molecular species by reversed-phase HPLC within 90 min, using a gradient of acetonitrile-methanol and ammonium acetate. A major feature of the method is the complete resolution of the stearoyl species, 18:0/20:4 and 18:0/22:6, at ambient temperature. By determining the amount of radioactivity incorporated into each fraction, the metabolism of individual molecular species of PS, and also of PE, labeled in the polar group can be investigated.
本文介绍了一种反相高效液相色谱法，用于监测放射性标记前体掺入磷脂酰丝氨酸（PS）各个多不饱和分子种类的极性基团中的情况。极性基团标记的PS经脱羧后，随后转化为三硝基苯基磷脂酰乙醇胺（Tnp-PE），使用乙腈-甲醇和醋酸铵梯度，在90分钟内通过反相高效液相色谱将其分离为各个分子种类。该方法的一个主要特点是在环境温度下能完全分离硬脂酰种类，即18:0/20:4和18:0/22:6。通过测定掺入每个馏分中的放射性活度，可以研究极性基团标记的PS以及PE各个分子种类的代谢情况。
