Meulemans A, Delsenne F
Faculté de médecine Xavier-Bichat, Laboratoire de Biophysique, Paris, France.
J Chromatogr B Biomed Appl. 1994 Oct 14;660(2):401-4. doi: 10.1016/0378-4347(94)00310-6.
Nitrite is one of the products of NO-synthase in biological media. It is slowly oxidized in animals to nitrate. We developed a simple and rapid method to determine simultaneously nitrite and nitrate in biological samples. Capillary ion electrophoresis with direct UV detection at 214 nm was used employing a carrier electrolyte consisting of 10 mM sodium sulfate and an electroosmotic flow modifier. The detection limit in ultrafiltrates of plasma, urine and brain tissue extracts was 25 ng/ml for both compounds. Nitrate levels in human plasma and urine were in the microgram/ml range. Nitrite could not be detected. Rat brain tissue extracts contained detectable amounts of nitrite and nitrate.
亚硝酸盐是生物介质中一氧化氮合酶的产物之一。它在动物体内会缓慢氧化为硝酸盐。我们开发了一种简单快速的方法来同时测定生物样品中的亚硝酸盐和硝酸盐。采用由10 mM硫酸钠和电渗流改性剂组成的载体电解质，在214 nm处进行直接紫外检测的毛细管离子电泳法。血浆、尿液和脑组织提取物超滤液中这两种化合物的检测限均为25 ng/ml。人血浆和尿液中的硝酸盐水平在微克/毫升范围内。未检测到亚硝酸盐。大鼠脑组织提取物中含有可检测量的亚硝酸盐和硝酸盐。
