Espinel-Ingroff A, Steele-Moore L
Medical College of Virginia/Virginia Commonwealth University, Richmond 23298-0049.
Diagn Microbiol Infect Dis. 1994 Oct;20(2):87-91. doi: 10.1016/0732-8893(94)90097-3.
This study aimed to compare the susceptibilities of fluconazole, obtained in two laboratories, using three RPMI-1640 formulations (manufacturers') and inhibition standards (80%). A total of 39 selected Candida species (in vitro susceptible and resistant) and Cryptococcus neoformans isolates were tested in each medium by broth macro- and microdilution procedures following the National Committee for Clinical Laboratory Standards proposed reference method (M27-P). Macrodilution minimum inhibitory concentrations (MIC80%) were the lowest drug concentrations with turbidity (growth) less than or equal to that of the specific 80% inhibition standards (1:5 growth control). Microdilution MIC-2 were the lowest drug concentrations in which there was prominent decrease in growth. In general, the three formulations of RPMI-1640 medium provided similar MICs for most of the yeasts tested in both laboratories and by each test.
本研究旨在比较在两个实验室中获得的氟康唑对三种RPMI-1640培养基配方（制造商提供）以及抑制标准（80%）的敏感性。按照美国国家临床实验室标准委员会提出的参考方法（M27-P），通过肉汤宏稀释法和微量稀释法，在每种培养基中对总共39株选定的念珠菌属菌种（体外敏感和耐药）以及新型隐球菌分离株进行了检测。宏稀释最低抑菌浓度（MIC80%）是指浊度（生长）小于或等于特定80%抑制标准（1:5生长对照）时的最低药物浓度。微量稀释MIC-2是指生长显著下降时的最低药物浓度。总体而言，对于两个实验室中通过每种检测方法测试的大多数酵母，三种RPMI-1640培养基配方提供了相似的MIC值。
