[3H]7-叠氮基-4-异丙基吖啶酮标记牛线粒体ADP/ATP载体蛋白的半胱氨酸159位点。
[3H]7-azido-4-isopropylacridone labels Cys159 of the bovine mitochondrial ADP/ATP-carrier protein.
作者信息
Oettmeier W, Masson K, Kalinna S
机构信息
Lehrstuhl Biochemie der Pflanzen, Ruhr-Universität, Bochum, Germany.
出版信息
Eur J Biochem. 1995 Feb 1;227(3):730-3. doi: 10.1111/j.1432-1033.1995.tb20195.x.
Acridones (9-azaanthracen-10-ones), especially halogen-substituted acridone-carboxylic acids are efficient inhibitors of [14C]ADP binding to the bovine mitochondrial ADP/ATP-carrier protein. 7-Iodoacridone-4-carboxylic acid displaces [14C]ADP in a competitive manner, which indicates that both compounds share an identical binding site. Upon ultraviolet illumination, 7-azido-4-[1',2'-3H]isopropyl-acridone binds covalently to the ADP/ATP-carrier protein. By sequencing of its proteolytic fragments, radioactivity was detected in Cys159. This amino acid is located close to Lys162, a target of 2-azido-[alpha-32P]ADP [Dalbon, P., Brandolin, G., Boulay, F., Hoppe, J. & Vignais, P. V. (1988) Biochemistry 27, 5141-5149].
吖啶酮（9-氮杂蒽-10-酮），尤其是卤代吖啶酮羧酸，是[14C]ADP与牛线粒体ADP/ATP载体蛋白结合的有效抑制剂。7-碘吖啶酮-4-羧酸以竞争性方式取代[14C]ADP，这表明两种化合物共享相同的结合位点。在紫外线照射下，7-叠氮基-4-[1',2'-3H]异丙基吖啶酮与ADP/ATP载体蛋白共价结合。通过对其蛋白水解片段进行测序，在Cys159中检测到放射性。该氨基酸位于靠近Lys162的位置，Lys162是2-叠氮基-[α-32P]ADP的作用靶点[达尔邦，P.，布兰多林，G.，布莱，F.，霍普，J. & 维尼亚斯，P. V.（1988年）《生物化学》27，5141 - 5149]。
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