新鲜分离的兔破骨细胞中细胞外钙离子对内向整流钾电流的抑制作用。
Inhibition of inwardly rectifying K+ current by external Ca2+ ions in freshly isolated rabbit osteoclasts.
作者信息
Yamashita N, Ishii T, Ogata E, Matsumoto T
机构信息
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
出版信息
J Physiol. 1994 Oct 15;480 ( Pt 2)(Pt 2):217-24. doi: 10.1113/jphysiol.1994.sp020354.
Abstract
- Regulation of membrane potential by extracellular Ca2+ concentration ([Ca2+]o) was examined in freshly isolated rabbit osteoclasts. 2. The resting membrane potential of osteoclasts was close to the K+ equilibrium potential in 1 mM Ca2+ medium. An elevation of [Ca2+]o caused membrane depolarization, accompanied by a decrease in the membrane conductance. 3. The inwardly rectifying K+ current observed under voltage clamp was dose-dependently inhibited by an elevation of [Ca2+]o, which explained the membrane depolarization caused by high [Ca2+]o. 4. Other divalent cations also inhibited the inwardly rectifying K+ current with the following order of potency: Ca2+ < Ni2+ < or = Co2+ < Cd2+. 5. In the presence of intracellular GTP gamma S the inwardly rectifying K+ current was irreversibly inhibited by [Ca2+]o, whereas the inhibition of the inwardly rectifying K+ current was greatly attenuated by intracellular application of GDP beta S. 6. Pertussis toxin (PTX) treatment did not abolish the inhibition of the inwardly rectifying K+ current caused by [Ca2+]o. 7. These results suggest that inwardly rectifying K+ channels in osteoclasts were regulated by a PTX-insensitive G-protein, which was coupled to the putative Ca2+ receptor or sensor on the cell membrane.
摘要
- 在新鲜分离的兔破骨细胞中研究了细胞外钙离子浓度（[Ca2+]o）对膜电位的调节作用。2. 在1 mM Ca2+ 培养基中，破骨细胞的静息膜电位接近钾离子平衡电位。[Ca2+]o升高导致膜去极化，同时膜电导降低。3. 在电压钳制下观察到的内向整流钾电流被[Ca2+]o升高剂量依赖性抑制，这解释了高[Ca2+]o引起的膜去极化。4. 其他二价阳离子也抑制内向整流钾电流，其效力顺序如下：Ca2+ < Ni2+ < 或 = Co2+ < Cd2+。5. 在细胞内存在GTPγS的情况下，内向整流钾电流被[Ca2+]o不可逆抑制，而通过细胞内应用GDPβS可大大减弱内向整流钾电流的抑制作用。6. 百日咳毒素（PTX）处理并未消除[Ca2+]o对内向整流钾电流的抑制作用。7. 这些结果表明，破骨细胞中的内向整流钾通道受一种对PTX不敏感的G蛋白调节，该G蛋白与细胞膜上假定的Ca2+受体或传感器偶联。
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