Nishi J, Miyanohara H, Nakajima T, Kitajima I, Yoshinaga M, Maruyama I, Miyata K
Department of Pediatrics, Kagoshima University.
Rinsho Byori. 1994 Dec;42(12):1227-33.
We used a method for molecular typing of the methicillin resistance determinant (mec) based on the size of the mec-associated hypervariable region amplified by the polymerase chain reaction (PCR) to examine 106 methicillin-resistant Staphylococcus aureus (MRSA), 9 methicillin-resistant (Mcr) S. epidermidis and 5 Mcr S. haemolyticus clinical isolates. In the 106 MRSA isolates, 5 sizes of PCR products were observed. The MRSA isolates were grouped into five hypervariable region (HVR) genotypes on the basis of the size of the PCR product. The PCR products amplified from 8 of 9 Mcr S. epidermidis isolates were the same as products amplified from MRSA isolates, which was confirmed by the PCR-SSCP (single-strand conformation polymorphism) method. In 32 methicillin-susceptible isolates, the target gene was not amplified. This method is thought to be useful in epidemiological investigations of nosocomial infections caused by MRSA. This is the first typing method capable of comparing the mec determinants of MRSA isolates and Mcr CNS isolates to establish the origin of the mec determinant.
我们采用一种基于聚合酶链反应（PCR）扩增的与mec相关的高变区大小的甲氧西林耐药决定簇（mec）分子分型方法，对106株耐甲氧西林金黄色葡萄球菌（MRSA）、9株耐甲氧西林表皮葡萄球菌（Mcr）和5株溶血葡萄球菌临床分离株进行检测。在106株MRSA分离株中，观察到5种PCR产物大小。根据PCR产物大小，将MRSA分离株分为5种高变区（HVR）基因型。9株Mcr表皮葡萄球菌分离株中有8株的PCR产物与MRSA分离株的产物相同，这通过PCR-SSCP（单链构象多态性）方法得以证实。在32株甲氧西林敏感分离株中，未扩增出目标基因。该方法被认为在由MRSA引起的医院感染的流行病学调查中有用。这是第一种能够比较MRSA分离株和Mcr凝固酶阴性葡萄球菌（CNS）分离株中的mec决定簇以确定mec决定簇来源的分型方法。
