Floss H G, Schleicher E, Potts R
J Biol Chem. 1976 Sep 25;251(18):5478-82.
The enzymatic preparation of (2S, 3R)- and (2S, 3S)-[3-3H] serine from the corresponding stereospecifically tritiated 3-P-glycerates is described. Following conversion into T-acetylserine, these substrates were used to establish the steric course of the O-acetylserine sulfhydrase reaction. The two samples of cysteine formed in this reaction were analyzed for their configuration at C-3. The results indicate that the replacement of the acetoxy by a sulfhydryl group in the O-acetylserine sulfhydrase reaction occurs with retention of configuration at carbon atom 3.
描述了从相应的立体定向氚化3-磷酸甘油酸酶促制备(2S, 3R)-和(2S, 3S)-[3-³H]丝氨酸的方法。在转化为O-乙酰丝氨酸后，这些底物被用于确定O-乙酰丝氨酸巯基酶反应的立体化学过程。分析了该反应中形成的两个半胱氨酸样品在C-3位的构型。结果表明，在O-乙酰丝氨酸巯基酶反应中，乙酰氧基被巯基取代时，碳原子3处的构型保持不变。
