Craven R B, Wenzel R P, Calhoun A M, Hendley J O, Hamory B H, Gwaltney J M
J Clin Microbiol. 1976 Sep;4(3):225-6. doi: 10.1128/jcm.4.3.225-226.1976.
Throat swab specimens from 1,494 Marine Corps recruits were inoculated into vials containing diphasic (broth/agar) mycoplasma medium and also onto PPLO agar plates, which were subsequently overlayed with sheep erythrocytes in saline agar. Strains of Mycoplasma pneumoniae were isolated from 89 (6%) of the specimens by one or both of the methods. Eight-one of the 89 (91%) positive specimens were cultured with diphasic medium, whereas only 42 (47%) were cultured with the overlay method (x2 = 7.44, P less than 0.01). The diphasic system for isolation of M. pneumoniae should be the method of choice in clinical and research application.
从1494名海军陆战队新兵采集咽喉拭子标本，接种于含有双相（肉汤/琼脂）支原体培养基的小瓶中，同时接种于PPLO琼脂平板上，随后在盐水中琼脂上覆盖绵羊红细胞。通过其中一种或两种方法，从89份（6%）标本中分离出肺炎支原体菌株。89份阳性标本中的81份（91%）用双相培养基培养，而用覆盖法培养的仅42份（47%）（χ² = 7.44，P<0.01）。用于分离肺炎支原体的双相系统应是临床和研究应用中的首选方法。
