Salinas J, Sanchez J, Buendia A J, Souriau A, Rodolakis A, Bernabé A, Cuello F
Dept. Patología Animal (Microbiología e Immunología), Fac. de Veterinaria, Univ. de Murcia, Spain.
Res Microbiol. 1994 Oct;145(8):611-20. doi: 10.1016/0923-2508(94)90078-7.
Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infected with the strain, were performed. Immunogold labelling was observed on the surface of reticular bodies (RB), but not on elementary bodies (EB). Immunolabelling was observed in ultrathin sections on both sides of the external chlamydial membrane, mainly on the inner side of EB and on the outer side of RB. Immunogold density was higher in EB than in RB; however, the absolute number of gold particles was higher in RB than EB, suggesting a loss of immunolabelling during the transformation of RB into EB. Specific labelling of LPS was also found in electrodense and adielectronic vacuoles near the surface of the cytoplasmic membrane of infected McCoy cells. These results suggest that the lack of protection against some chlamydial strains, despite the presence of anti-LPS specific antibodies, is due to the localization of LPS on the inner side of the external membrane of EB.
使用四种抗衣原体脂多糖（LPS）的单克隆抗体，通过免疫电子显微镜研究它们在鹦鹉热衣原体AB7流产致病菌株中的定位和分布。对整个衣原体采用非包埋技术，并对感染该菌株的 McCoy 细胞采用包埋后技术。在网状体（RB）表面观察到免疫金标记，但在原体（EB）表面未观察到。在超薄切片中，在衣原体外部膜两侧均观察到免疫标记，主要在 EB 的内侧和 RB 的外侧。EB 中的免疫金密度高于 RB；然而，RB 中的金颗粒绝对数量高于 EB，这表明在 RB 向 EB 转化过程中免疫标记有所丢失。在感染的 McCoy 细胞质膜表面附近的电子致密和非电子致密液泡中也发现了 LPS 的特异性标记。这些结果表明，尽管存在抗 LPS 特异性抗体，但对某些衣原体菌株缺乏保护作用是由于 LPS 位于 EB 外部膜的内侧。
