通过酶联免疫吸附测定（ELISA）检测血清IgM类风湿因子可明确血清阴性幼年类风湿关节炎中关节变形疾病患者的一个亚组。
Serum IgM rheumatoid factor by enzyme-linked immunosorbent assay (ELISA) delineates a subset of patients with deforming joint disease in seronegative juvenile rheumatoid arthritis.
作者信息
Aggarwal A, Dabadghao S, Naik S, Misra R
机构信息
Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
出版信息
Rheumatol Int. 1994;14(4):135-8. doi: 10.1007/BF00579698.
Using human IgG as an antigen in an enzyme-linked immunosorbent assay (ELISA), we looked for the presence of IgM rheumatoid factor (RF) in the sera of 74 children with juvenile rheumatoid arthritis (JRA). Nine children had RF detectable by both latex agglutination and ELISA. Forty-five percent (26 of 65) of the children who were seronegative by latex agglutination were found to be positive for IgM RF by ELISA. The prevalence of IgM RF was higher in patients with polyarticular onset disease (57.4%) than in those with pauciarticular onset (38.5%) or systemic onset (27.2%) disease. The prevalence of RF was higher in sera from patients with deforming joint disease than those without deformities (P < 0.01).
在酶联免疫吸附测定（ELISA）中，我们以人IgG作为抗原，检测了74例幼年类风湿性关节炎（JRA）患儿血清中IgM类风湿因子（RF）的存在情况。9例患儿通过乳胶凝集试验和ELISA均可检测到RF。乳胶凝集试验血清学阴性的患儿中，45%（65例中的26例）通过ELISA检测发现IgM RF呈阳性。多关节起病型疾病患者中IgM RF的患病率（57.4%）高于少关节起病型（38.5%）或全身起病型（27.2%）疾病患者。关节变形疾病患者血清中RF的患病率高于无关节畸形患者（P < 0.01）。
Zotero 插件