Roizenblatt S, Goldenberg J, Gabriel A, Hilário M O, Atra E
Department of Medicine, Escola Paulista de Medicina, São Paulo, Brazil.
Allergol Immunopathol (Madr). 1993 Sep-Oct;21(5):197-200.
Hidden 19S IgM rheumatoid factor (RF) and 7S IgG RF were assayed in 24 children with juvenile chronic arthritis (JCA) and 26 controls, in whole sera and in the IgM and IgG containing fractions of the serum after separation by acid gel filtration. When non-fractioned serum was used, IgM RF detection was positive in 4.2% by latex fixation test and 4.2% by ELISA. IgG RF was positive in 91.7% by ELISA. In the IgM containing fraction of the serum, the positivity for IgM RF was 41.7% by LFT and 54.2% by ELISA. In the IgG containing fraction the positivity for IgG RF was 100%. These results suggest toward the need of previous serum dissociation before hidden 19S IgM RF is assayed and demonstrate high prevalence of 7S IgG RF.
在24例幼年慢性关节炎（JCA）患儿和26例对照中，采用酸凝胶过滤分离血清后，对全血清以及血清中含IgM和IgG的组分检测隐匿性19S IgM类风湿因子（RF）和7S IgG RF。当使用未分离血清时，乳胶凝集试验检测IgM RF阳性率为4.2%，ELISA法为4.2%。ELISA法检测IgG RF阳性率为91.7%。在血清中含IgM的组分中，乳胶凝集试验检测IgM RF阳性率为41.7%，ELISA法为54.2%。在血清中含IgG的组分中，IgG RF阳性率为100%。这些结果表明，在检测隐匿性19S IgM RF之前需要先对血清进行解离，并证明7S IgG RF的高患病率。
