Ma J Y, Borch K, Sjöstrand S E, Janzon L, Mårdh S
Dept. of Cell Biology, Faculty of Health Sciences, Linköping University, Sweden.
Scand J Gastroenterol. 1994 Nov;29(11):961-5. doi: 10.3109/00365529409094870.
Helicobacter pylori is a major cause of gastritis, and the parietal cell H,K-adenosine triphosphatase (ATPase) is a major autoantigen in autoimmune atrophic corpus gastritis, which may eventually lead to pernicious anemia and/or neuropathy. Whether the bacterium induces the autoimmune response is unknown.
By means of enzyme-linked immunosorbent assay the occurrence of antibodies against porcine H,K-ATPase and H. pylori was determined in sera from 30 patients with pernicious anemia.
All sera scored positive against H,K-ATPase, and 25 (83%) scored positive against H. pylori. The titers of antibodies against both antigen preparations inversely correlated with the duration of disease. A possible common epitope in the antigen preparations was tested with a competition assay. There was no indication of a common epitope in either human or porcine H,K-ATPase and H. pylori.
There was a positive correlation and a high incidence of antibodies against H,K-ATPase and H. pylori in sera from patients with pernicious anemia. These antibodies recognized different epitopes.
幽门螺杆菌是胃炎的主要病因，壁细胞H，K - 腺苷三磷酸酶（ATP酶）是自身免疫性萎缩性胃体胃炎的主要自身抗原，最终可能导致恶性贫血和/或神经病变。该细菌是否诱导自身免疫反应尚不清楚。
通过酶联免疫吸附测定法，测定了30例恶性贫血患者血清中抗猪H，K - ATP酶和幽门螺杆菌抗体的出现情况。
所有血清对H，K - ATP酶检测呈阳性，25例（83%）对幽门螺杆菌检测呈阳性。针对两种抗原制剂的抗体滴度与病程呈负相关。用竞争试验检测了抗原制剂中可能存在的共同表位。在人或猪的H，K - ATP酶与幽门螺杆菌中均未显示存在共同表位。
恶性贫血患者血清中抗H，K - ATP酶和幽门螺杆菌抗体呈正相关且发生率高。这些抗体识别不同的表位。
