Grant R F, Windsor S K, Malinak C J, Bartz C R, Sabo A, Benveniste R E, Tsai C C
Regional Primate Research Center, University of Washington, Seattle 98195.
Virology. 1995 Feb 20;207(1):292-6. doi: 10.1006/viro.1995.1080.
Infectious virus resembling type D simian retrovirus (SRV) was isolated from Ethiopian baboons (Papio cynocephalus) (SRV-Pc) housed at the University of Washington Regional Primate Research Center. When baboon peripheral blood mononuclear cells (PBMC) or tissues were cocultured with the H-9 human T-cell line or the Raji human B-cell line, large multinucleated syncytia positive for SRV-2 antigens were observed microscopically. Immunoblot analysis of purified SRV-Pc from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV-2 serum. Fresh PBMC and cocultured cells were positive by polymerase chain reaction using two different sets of SRV-2 primers. Preliminary sequence analysis of two separate isolates from portions of the SRV-Pc p27 and gp20 regions revealed homology with SRV-1, SRV-2, and Mason-Pfizer monkey virus. The homologies in the p27 segment were 91-94% and the homologies in the gp20 segment were 72-75%.
从华盛顿大学灵长类动物研究中心饲养的埃塞俄比亚狒狒（Papio cynocephalus）中分离出了类似D型猴逆转录病毒（SRV）的传染性病毒（SRV-Pc）。当狒狒外周血单个核细胞（PBMC）或组织与H-9人T细胞系或Raji人B细胞系共培养时，在显微镜下观察到对SRV-2抗原呈阳性的大型多核巨细胞。对细胞培养上清液中纯化的SRV-Pc进行免疫印迹分析表明，病毒核心蛋白和包膜蛋白与兔抗SRV-2血清发生反应。使用两组不同的SRV-2引物通过聚合酶链反应检测，新鲜PBMC和共培养细胞呈阳性。对来自SRV-Pc p27和gp20区域部分的两个单独分离株进行的初步序列分析显示，其与SRV-1、SRV-2和梅森- Pfizer猴病毒具有同源性。p27片段中的同源性为91-94%，gp20片段中的同源性为72-75%。
