Adkins S, Quadt R, Choi T J, Ahlquist P, German T
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Virology. 1995 Feb 20;207(1):308-11. doi: 10.1006/viro.1995.1083.
An RNA-dependent RNA polymerase activity was found associated with virions of tomato spotted wilt virus (TSWV), a plant- and insect-infecting member of the family Bunyaviridae. Radiolabeled nucleoside triphosphates were incorporated into trichloroacetic acid-precipitable products by detergent-disrupted, purified TSWV virions. Incorporation was reduced to near-background levels when RNase was present in the reaction mixture. The predominantly double-stranded RNA products were RNase-resistant at high but not low salt concentrations. The activity required manganese and was independent of a DNA template. Discrete products of approximately 3.0 kb and heterogeneous smaller products were synthesized that hybridized to purified TSWV RNA and transcripts of cDNA clones encompassing parts of each of the three genomic RNAs. The predominant products were viral sense although significant amounts of viral complementary sense S RNA products were also synthesized.
在番茄斑萎病毒（TSWV）的病毒粒子中发现了一种依赖RNA的RNA聚合酶活性，TSWV是布尼亚病毒科的一种感染植物和昆虫的病毒。经去污剂处理的纯化TSWV病毒粒子将放射性标记的核苷三磷酸掺入三氯乙酸可沉淀产物中。当反应混合物中存在核糖核酸酶时，掺入量降至接近背景水平。主要的双链RNA产物在高盐浓度下对核糖核酸酶有抗性，但在低盐浓度下则不然。该活性需要锰，且不依赖于DNA模板。合成了约3.0 kb的离散产物和异质的较小产物，它们与纯化的TSWV RNA以及包含三个基因组RNA各自部分的cDNA克隆转录本杂交。主要产物是病毒正义链，尽管也合成了大量的病毒互补反义链S RNA产物。
