Rauth S, Kichina J, Green A, Bratescu L, Das Gupta T K
Department of Surgical Oncology, University of Illinois at Chicago 60612.
Anticancer Res. 1994 Nov-Dec;14(6B):2457-63.
Nine human melanoma cell lines established in our laboratory were analyzed for p53 gene expression and their tumorigenic and metastatic potential in nude mice. Northern blot analyses showed that five of the cell lines (55%) had either complete loss or low levels of p53 transcripts. Immunocytochemical analysis for p53 protein expression agreed with mRNA analysis results. Nucleotide sequencing showed no mutations in exons 5 through 8 of the gene. All cell lines except one gave rise to tumors at subcutaneous inoculation sites in nude mice. The melanoma cell line UISO-MEL-6, completely lacking p53 expression, spontaneously metastasized to lung and liver in nude mice.
对我们实验室建立的9种人黑色素瘤细胞系进行了p53基因表达分析及其在裸鼠中的致瘤和转移潜能分析。Northern印迹分析表明，其中5种细胞系（55%）要么完全缺失p53转录本，要么p53转录本水平较低。p53蛋白表达的免疫细胞化学分析结果与mRNA分析结果一致。核苷酸测序显示该基因的外显子5至8没有突变。除一种细胞系外，所有细胞系在裸鼠皮下接种部位均形成肿瘤。完全缺乏p53表达的黑色素瘤细胞系UISO-MEL-6在裸鼠中自发转移至肺和肝。
