Maxwell S A
Department of Thoracic and Cardiovascular Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Anticancer Res. 1994 Nov-Dec;14(6B):2549-56.
The p53 gene product suppresses both tumor cell growth and the cellular transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many gene expression events are involved in p53-mediated growth arrest and apoptosis. Two-dimensional gel electrophoresis was employed to investigate changes in whole-cell protein expression in p53-null H1299 cells that were transfected with a recombinant adenovirus expressing wild-type p53 at high efficiency. The two-dimensional gel analysis was restricted to proteins ranging in mass from 12,000 to 80,000 daltons. A total of 17 proteins were induced and one was repressed within 16 h of expression of exogenous wild-type p53 in H1299 cells. These results indicate that p53-mediated growth suppression involves a complex array of gene expression events.
p53基因产物既能抑制肿瘤细胞生长，又能抑制癌基因促进的细胞转化过程。虽然已知有几个基因受p53正向调控，但尚不清楚p53介导的生长停滞和凋亡涉及多少基因表达事件。采用二维凝胶电泳技术，研究了用高效表达野生型p53的重组腺病毒转染的p53基因缺失的H1299细胞全细胞蛋白质表达的变化。二维凝胶分析仅限于质量范围在12,000至80,000道尔顿之间的蛋白质。在H1299细胞中外源野生型p53表达的16小时内，共有17种蛋白质被诱导，1种蛋白质被抑制。这些结果表明，p53介导的生长抑制涉及一系列复杂的基因表达事件。
