Chamlian A, Benkoël L, Ikoli J F, Brisse J, Jacob T
Laboratoire d'Anatomie Pathologique et de Cytochimie Hépatique, Faculté de Médecine, Marseille, France.
Cell Mol Biol (Noisy-le-grand). 1993 May;39(3):243-51.
Immunohistochemical methods have been used to localize an HCV antigen on paraffin embedded liver tissue sections by means of monoclonal antibodies to C100-3 nonstructural protein. Peroxidase-antiperoxidase, alkaline phosphatase-antialkaline phosphatase, biotin-streptavidin-peroxidase and immunogold silver staining methods showed a nuclear staining of the hepatocytes in cases of chronic hepatitis with positive HCV serology, alcoholic liver disease and hepatocarcinoma. No cross reactions were observed with viral hepatitis B and delta antigens. The strongest reaction without background staining was obtained with immunogold silver staining. Nuclear localization was compared to the cytoplasmic staining described in the literature.
