Nakajima T, Yamashita N, Matsui H, Suzuki N, Kaneoka H, Mizushima Y, Sakane T
Institute of Medical Science, St Marianna University, Kanagawa, Japan.
Br J Haematol. 1995 Feb;89(2):258-65. doi: 10.1111/j.1365-2141.1995.tb03298.x.
Human myeloid leukaemia cell lines have been shown to differentiate into distinct cell lineages in vitro in response to several differentiation-inducing agents. A human eosinophilic leukaemia cell line, EoL-1, has been shown to differentiate into mature eosinophilic granulocytes by treatment with the culture supernatant of a human T-cell line, HIL-3. In this study we have studied whether the EoL-1 cell line has potential to differentiate into cell lineage other than eosinophils. We found that EoL-1 cells cultured in the presence of tumour necrosis factor (TNF)-alpha (10 u/ml) and interferon (IFN)-gamma (1000 u/ml) for 2-4 d differentiated into macrophage-like cells in morphology, and expressed CD14 antigen on their cell surface. It is possible that the small subpopulation of EoL-1 cells which contains non-specific esterase (NSE) activity may be preferentially differentiated by TNF-alpha and IFN-gamma. To clarify this issue, we have cloned the EoL-1 cell line and obtained NSE negative and positive sublines. Both EoL-1 sublines differentiated into monocyte/macrophage-like cells, because: (a) EoL-1 sublines were induced to express CD14 antigen, and (b) they attached firmly to the plastic wells; (c) after differentiation they became strongly positive for NSE staining, and secreted TNF-alpha in response to the stimulation with lipopolysaccharide; and (d) they exhibited potent phagocytic activity. Therefore, we found that the EoL-1 cell line has the ability to differentiate not only into mature eosinophilic cells but also into monocyte/macrophage cell lineage, suggesting that EoL-1 cells represent immature cells with ability to differentiate into multiple cell lineages.
已表明人类髓系白血病细胞系在体外对几种分化诱导剂有反应时可分化为不同的细胞谱系。一种人类嗜酸性粒细胞白血病细胞系EoL-1，已被证明通过用人T细胞系HIL-3的培养上清液处理可分化为成熟的嗜酸性粒细胞。在本研究中，我们研究了EoL-1细胞系是否有潜力分化为除嗜酸性粒细胞以外的细胞谱系。我们发现，在肿瘤坏死因子（TNF）-α（10 U/ml）和干扰素（IFN）-γ（1000 U/ml）存在下培养2-4天的EoL-1细胞在形态上分化为巨噬细胞样细胞，并在其细胞表面表达CD14抗原。含有非特异性酯酶（NSE）活性的EoL-1细胞的小亚群可能优先被TNF-α和IFN-γ分化。为了阐明这个问题，我们克隆了EoL-1细胞系并获得了NSE阴性和阳性亚系。两个EoL-1亚系都分化为单核细胞/巨噬细胞样细胞，原因如下：（a）EoL-1亚系被诱导表达CD14抗原；（b）它们牢固地附着在塑料孔上；（c）分化后它们对NSE染色呈强阳性，并在脂多糖刺激下分泌TNF-α；（d）它们表现出强大的吞噬活性。因此，我们发现EoL-1细胞系不仅有能力分化为成熟的嗜酸性细胞，而且有能力分化为单核细胞/巨噬细胞谱系，这表明EoL-1细胞代表具有分化为多种细胞谱系能力的未成熟细胞。
