Matsumura K, Kawamoto K
Department of Neurosurgery, Kansai Medical College.
Hum Cell. 1994 Sep;7(3):158-66.
The history of brain tumor cultures is old. Established cell lines of brain tumors have increased in recent years, but reports concerning the results of these cultures are few. The results of cultures of gliomas (103 cases of astrocytoma, 9 of ependymoma, 2 of oligodendroglioma and others) obtained from 117 patients treated at our department between 1979 and 1993 were evaluated. The morphological characteristic of these glioma cells were studied, and their cellular kinetics were investigated by flow cytometry. Except for 5 cases, all tumors grew on the flask in the primary cultures, indicating that gliomas were readily cultured. Differences in morphological characteristics and cellular kinetics were not observed in cultures which had been maintained for more than 6 months in astrocytomas of grades II, III, and IV. Cells lines were successively established in 3 cases of glioblastoma and 2 of ependymoma. The results of FCM revealed that those cells which grew well on cultures had high proliferative indices.
脑肿瘤培养的历史悠久。近年来，已建立的脑肿瘤细胞系有所增加，但关于这些培养结果的报告却很少。我们评估了1979年至1993年间在我科接受治疗的117例患者所获得的胶质瘤（103例星形细胞瘤、9例室管膜瘤、2例少突胶质细胞瘤等）培养结果。研究了这些胶质瘤细胞的形态特征，并通过流式细胞术研究了它们的细胞动力学。除5例肿瘤外，所有肿瘤在原代培养中均在培养瓶中生长，表明胶质瘤易于培养。在II、III和IV级星形细胞瘤中，培养超过6个月的培养物未观察到形态特征和细胞动力学的差异。在3例胶质母细胞瘤和2例室管膜瘤中成功建立了细胞系。流式细胞术结果显示，在培养物中生长良好的细胞具有较高的增殖指数。
