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从花生植株茎中纯化凝集素。

Purification of lectins from the stems of peanut plants.

作者信息

Singh R, Das H R

机构信息

Lectin Research Laboratory, Centre for Biochemical Technology, Delhi, India.

出版信息

Glycoconj J. 1994 Aug;11(4):282-5. doi: 10.1007/BF00731199.

DOI:10.1007/BF00731199
PMID:7873922
Abstract

The stem of the peanut plant contains two lectins, a methyl alpha-mannoside specific lectin (SL-I) and a lactose/cellobiose specific lectin (SL-II). These lectins are found to be developmentally regulated and maximum activities are observed in 3-4-weeks-old plants. The two lectins SL-I and SL-II have been purified from 3-week-old stem by affinity chromatography on Sephadex G-50 and guar gum matrices respectively. Both are glycosylated lectins and have the identical subunit molecular weight of 31 kDa.

摘要

花生植株的茎含有两种凝集素,一种是甲基α-甘露糖苷特异性凝集素(SL-I),另一种是乳糖/纤维二糖特异性凝集素(SL-II)。这些凝集素在发育过程中受到调控,在3 - 4周龄的植株中观察到最大活性。两种凝集素SL-I和SL-II已分别通过在Sephadex G-50和瓜尔胶基质上的亲和层析从3周龄的茎中纯化出来。两者都是糖基化凝集素,具有相同的31 kDa亚基分子量。

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本文引用的文献

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Differences in properties of peanut seed lectin and purified galactose- and mannose-binding lectins from nodules of peanut.花生种子凝集素与花生根瘤中分离纯化的半乳糖和甘露糖结合凝集素性质的差异。
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芝麻菜茎凝集素的纯化和质谱鉴定。
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