从花生植株茎中纯化凝集素。
Purification of lectins from the stems of peanut plants.
作者信息
Singh R, Das H R
机构信息
Lectin Research Laboratory, Centre for Biochemical Technology, Delhi, India.
出版信息
Glycoconj J. 1994 Aug;11(4):282-5. doi: 10.1007/BF00731199.
The stem of the peanut plant contains two lectins, a methyl alpha-mannoside specific lectin (SL-I) and a lactose/cellobiose specific lectin (SL-II). These lectins are found to be developmentally regulated and maximum activities are observed in 3-4-weeks-old plants. The two lectins SL-I and SL-II have been purified from 3-week-old stem by affinity chromatography on Sephadex G-50 and guar gum matrices respectively. Both are glycosylated lectins and have the identical subunit molecular weight of 31 kDa.
花生植株的茎含有两种凝集素，一种是甲基α-甘露糖苷特异性凝集素（SL-I），另一种是乳糖/纤维二糖特异性凝集素（SL-II）。这些凝集素在发育过程中受到调控，在3 - 4周龄的植株中观察到最大活性。两种凝集素SL-I和SL-II已分别通过在Sephadex G-50和瓜尔胶基质上的亲和层析从3周龄的茎中纯化出来。两者都是糖基化凝集素，具有相同的31 kDa亚基分子量。
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