Cals M M, Guillomot M, Martin P
Lab. de Génétique biochimique et de Cytogénétique, Institut National de la Recherche Agronomique (INRA), Jouy-en-Josas, France.
Cell Mol Biol (Noisy-le-grand). 1994 Dec;40(8):1143-50.
The localization of lactoperoxidase (LP), which catalyzes in milk the peroxidation of endogenous thiocyanate in the presence of peroxide, was investigated in the goat lactating mammary gland, using immunofluorescence and hybridization techniques. In situ hybridization experiments have demonstrated the presence of LP mRNA within the cytoplasm of alveolar epithelial cells (acini) where LP has been detected by immunofluorescence labelling with anti-LP polyclonal antibodies. Taken together, these data provide the first direct evidence for expression of the LP gene within secretory cells of the lactating mammary gland.
利用免疫荧光和杂交技术，对山羊泌乳乳腺中催化乳中内源性硫氰酸盐在过氧化物存在下发生过氧化反应的乳过氧化物酶（LP）进行了定位研究。原位杂交实验已证明，在经抗LP多克隆抗体免疫荧光标记检测到LP的肺泡上皮细胞（腺泡）胞质内存在LP mRNA。综上所述，这些数据为泌乳乳腺分泌细胞中LP基因的表达提供了首个直接证据。
