梭菌神经毒素对神经递质释放的抑制作用与突触体蛋白的特异性蛋白水解有关。
Inhibition of neurotransmitter release by clostridial neurotoxins correlates with specific proteolysis of synaptosomal proteins.
作者信息
Blasi J, Binz T, Yamasaki S, Link E, Niemann H, Jahn R
机构信息
Departament de Biologia Cel.lular i Anatomia Patologica, Facultat de Medicina, Universitat de Barcelona, Spain.
出版信息
J Physiol Paris. 1994;88(4):235-41. doi: 10.1016/0928-4257(94)90086-8.
Rat brain synaptosomes were used to study the effect of several clostridial neurotoxins on the neurotransmitter release. In this system the blockade of transmitter release correlated with the proteolytic activity of the toxins. Blockade of glutamate release was linked to selective proteolysis of one of the following synaptic proteins: synaptobrevin (BoNT/D, BoNT/F); SNAP-25 (BoNT/A, BoNT/E), or HPC-1/syntaxin (BoNT/C1). All the toxins used had an inhibitory effect on synaptosomes with the exception of BoNT/F. BoNT/F cleaved synaptobrevin in permeabilized synaptosomes but failed to produce the same effect on intact synaptosomes.
大鼠脑突触体被用于研究几种梭菌神经毒素对神经递质释放的影响。在这个系统中，递质释放的阻断与毒素的蛋白水解活性相关。谷氨酸释放的阻断与以下突触蛋白之一的选择性蛋白水解有关：突触小泡蛋白（肉毒杆菌神经毒素D型、肉毒杆菌神经毒素F型）；突触融合蛋白25（肉毒杆菌神经毒素A型、肉毒杆菌神经毒素E型），或HPC-1/ syntaxin（肉毒杆菌神经毒素C1型）。除肉毒杆菌神经毒素F型外，所有使用的毒素对突触体都有抑制作用。肉毒杆菌神经毒素F型在通透的突触体中切割突触小泡蛋白，但对完整的突触体未能产生相同的效果。
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