Shiozaki K, Russell P
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Cell Mol Biol Res. 1994;40(3):241-3.
Protein phosphatase 2C (PP2C) is one of four major classes of protein serine/threonine-phosphatases, which requires Mg2+ for its activity. PP2C activity distributes ubiquitously among eukaryotes and enzymes purified from mammalian tissues were well characterized biochemically, however, very little is known about their biological function. We have been trying to identify cellular function of PP2C by genetic analyses in fission yeast Schizosaccharomyces pombe. So far, three PP2C homologs, ptc1+, ptc2+, and ptc3+, have been cloned and their activity were detected in the S. pombe cell lysates. Experiments using ptc mutants constructed by gene disruption technique have revealed the involvement of PP2C in the heat shock response and possibly osmoregulation in yeast cells.
蛋白磷酸酶2C（PP2C）是蛋白丝氨酸/苏氨酸磷酸酶的四大主要类别之一，其活性需要Mg2+。PP2C活性在真核生物中普遍分布，从哺乳动物组织中纯化的酶在生化方面得到了很好的表征，然而，关于它们的生物学功能却知之甚少。我们一直在尝试通过裂殖酵母粟酒裂殖酵母中的遗传分析来确定PP2C的细胞功能。到目前为止，已经克隆了三个PP2C同源物ptc1+、ptc2+和ptc3+，并在粟酒裂殖酵母细胞裂解物中检测到了它们的活性。使用通过基因破坏技术构建的ptc突变体进行的实验表明，PP2C参与了酵母细胞的热休克反应以及可能的渗透调节。
