Nagao A T, Mai F H, Pereira A B, Carneiro-Sampaio M M
Department of Immunology, Universidade de Saõ Paulo, Brazil.
J Investig Allergol Clin Immunol. 1994 Sep-Oct;4(5):234-7.
We measured salivary, urinary and fecal secretory IgA (sIgA) levels in 11 children with total IgA deficiency and in 6 children with partial IgA deficiency using an ELISA technique. This was based on flexible microplates coated with antisecretory component (SC) and peroxidase-conjugated anti-IgA as a second antibody. Selective IgA deficiency is diagnosed as a serum IgA concentration < or = 0.05 g/l; partial IgA deficiency is diagnosed as a serum concentration of IgA > 0.05 g/l but 2 SD below normal levels. No salivary or fecal sIgA, and only low levels of urinary sIgA, were detected in the selective IgA-deficient group. The partial IgA-deficient children presented with low levels of salivary, urinary and fecal sIgA. Fecal sIgA levels correlated with salivary sIgA levels (p < 0.01) but not with urinary sIgA levels (p > 0.05) in the IgA-deficient patients. We found that all the children with partial IgA deficiency, except one, had detectable, but low values of secretory IgA. Our data suggest that these patients also have a partial mucosal IgA deficiency.
我们采用酶联免疫吸附测定（ELISA）技术，对11名全IgA缺乏儿童和6名部分IgA缺乏儿童的唾液、尿液和粪便分泌型IgA（sIgA）水平进行了测量。该检测基于包被抗分泌成分（SC）的弹性微孔板，以及作为第二抗体的过氧化物酶偶联抗IgA。选择性IgA缺乏症的诊断标准为血清IgA浓度≤0.05g/l；部分IgA缺乏症的诊断标准为血清IgA浓度＞0.05g/l但低于正常水平2个标准差。在选择性IgA缺乏组中，未检测到唾液或粪便中的sIgA，仅检测到低水平的尿液sIgA。部分IgA缺乏的儿童唾液、尿液和粪便中的sIgA水平较低。在IgA缺乏患者中，粪便sIgA水平与唾液sIgA水平相关（p＜0.01），但与尿液sIgA水平无关（p＞0.05）。我们发现，除1名患者外，所有部分IgA缺乏的儿童均可检测到分泌型IgA，但水平较低。我们的数据表明，这些患者也存在部分黏膜IgA缺乏。
