Bioaccumulation and toxic effects of elevated levels of 3,3',4,4'-tetrachloroazobenzene (33'44'-TCAB) towards aquatic organisms. I: A simple method for the rapid extraction, detection and determination of 33'44'-TCAB in multiple biological samples.

The polychlorinated aromatic compound 3,3',4,4'-tetrachloroazobenzene (33'44'-TCAB) is a contaminant of 3,4-dichloroaniline based herbicides, but there have been no reports of methods for the quantitative determination of 33'44'-TCAB in biological organisms. In this communication we address this knowledge gap by presenting a method for the rapid extraction, detection and determination of 33'44'-TCAB in multiple biological samples based on the alkaline decomposition of the sample followed by alumina cartridge column chromatography and GC-HRMS analysis.