Jia H G, Rao Z R, Shi J W
Department of Anatomy, Fourth Military Medical University, Shaanxi, People's Republic of China.
Brain Res. 1994 Nov 14;663(2):181-90. doi: 10.1016/0006-8993(94)91262-9.
The morphological basis of how visceral information from the nucleus of the solitary tract (NTS) is relayed from the parabrachial nucleus (PBN) to the central nucleus of the amygdala (Ce) was studied at the light and electron microscopic levels using the anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L), kainic acid degeneration, and retrograde tracing with horseradish peroxidase (HRP). After injection of PHA-L into the caudal NTS, anterogradely labeled fibers and terminals were predominantly distributed in the external lateral (el) and central lateral (cl) subnuclei of the PBN. After injection of HRP into the Ce, retrogradely labeled neurons in PBN were mainly distributed in the same areas. In double-labeling experiments, there was a clear overlap between neuronal elements labeled with HRP and PHA-L in the el and cl. At the electron microscopic level, the PHA-L-labeled axon terminals from the NTS mainly contained spherical agranular synaptic vesicles and formed asymmetric contacts with the postsynaptic dendrites or dendritic spines in PBN. After the lesioning agent kainic acid was injected into the NTS and HRP deposited in the Ce, it was found the afferent fibers from the NTS made direct synaptic contact with the lateral PBN neurons which in turn projected to Ce. Such evidence adds to our growing knowledge of regulation of visceral function in central nervous system and would be likely helpful for understanding the important roles of the NTS, PBN and Ce in the central control of cardiovascular, respiratory and gastrointestinal functions.
运用顺行示踪剂菜豆白细胞凝集素（PHA-L）、 kainic 酸变性以及辣根过氧化物酶（HRP）逆行示踪技术，在光镜和电镜水平研究了孤束核（NTS）的内脏信息从臂旁核（PBN）传递至杏仁核中央核（Ce）的形态学基础。将PHA-L注入尾侧NTS后，顺行标记的纤维和终末主要分布于PBN的外侧（el）和中央外侧（cl）亚核。将HRP注入Ce后，PBN中逆行标记的神经元主要分布在相同区域。在双重标记实验中，el和cl中用HRP和PHA-L标记的神经元成分之间存在明显重叠。在电镜水平，来自NTS的PHA-L标记轴突终末主要含有球形无颗粒突触小泡，并与PBN中的突触后树突或树突棘形成不对称接触。在将损伤剂 kainic 酸注入NTS并将HRP注入Ce后，发现来自NTS的传入纤维与外侧PBN神经元直接形成突触联系，这些神经元进而投射至Ce。这些证据增加了我们对中枢神经系统内脏功能调节的认识，并可能有助于理解NTS、PBN和Ce在心血管、呼吸和胃肠功能中枢控制中的重要作用。
