大鼠迷走神经在体外缓慢轴突运输蛋白的释放

Release of slow axonally transported proteins from the rat vagus nerve in vitro.

作者信息

Remgård P, Kanje M

机构信息

Department of Animal Physiology, University of Lund, Sweden.

出版信息

Brain Res. 1994 Nov 14;663(2):326-8. doi: 10.1016/0006-8993(94)91280-7.

DOI:10.1016/0006-8993(94)91280-7

PMID:7874518

Abstract

The cultured rat vagus nerve was used to investigate the release of [35S]methionine-labelled slow axonally transported proteins during regeneration. After metabolic labelling the released proteins were collected from an isolated compartment at the distal end of the nerve. Several proteins were released at a time point consistent with the arrival of slow axonally transported proteins at the collection compartment, including actin and a group of 150 kDa proteins.

摘要

培养的大鼠迷走神经被用于研究再生过程中[35S]甲硫氨酸标记的慢速轴突运输蛋白的释放。代谢标记后，从神经远端的一个分离隔室收集释放的蛋白质。在与慢速轴突运输蛋白到达收集隔室相一致的时间点释放了几种蛋白质，包括肌动蛋白和一组150 kDa的蛋白质。