挥发性麻醉剂异氟烷对大鼠大脑皮层中钙依赖型谷氨酸释放的影响。

The effect of the volatile anesthetic isoflurane on Ca(2+)-dependent glutamate release from rat cerebral cortex.

作者信息

Larsen M, Grøndahl T O, Haugstad T S, Langmoen I A

机构信息

Institute for Surgical Research, Rikshospitalet, University of Oslo, Norway.

出版信息

Brain Res. 1994 Nov 14;663(2):335-7. doi: 10.1016/0006-8993(94)91282-3.

DOI:10.1016/0006-8993(94)91282-3

PMID:7874520

Abstract

A major effect of volatile anesthetics is to reduce excitatory synaptic transmission. In the present study the stimulated release of glutamate under the influence of increasing concentrations of isoflurane was studied in vitro by utilizing hippocampal slices from Wistar rats. Ca(2+)-dependent release was calculated by subtracting stimulated release with blocked synaptic transmission (50 mM K+, 0 mM Ca2+ and 4 mM Mg2+) from total evoked release (50 mM K+, 2 mM Ca2+ and 1 mM Mg2+). Isoflurane 0.5, 1.5 and 3% reduced Ca(2+)-dependent release of glutamate to 69, 58 and 49%, respectively (P < 0.05 for all related to control). These results are in agreement with the possibility of reduced release of transmitter as a mechanism of action of volatile anesthetics.

摘要

挥发性麻醉剂的一个主要作用是减少兴奋性突触传递。在本研究中，通过使用Wistar大鼠的海马切片，在体外研究了异氟烷浓度增加影响下谷氨酸的刺激释放。通过从总诱发释放（50 mM K +、2 mM Ca2 +和1 mM Mg2 +）中减去突触传递被阻断时的刺激释放（50 mM K +、0 mM Ca2 +和4 mM Mg2 +）来计算钙依赖性释放。0.5%、1.5%和3%的异氟烷分别将谷氨酸的钙依赖性释放降低至69%、58%和49%（与对照组相比，所有P < 0.05）。这些结果与递质释放减少作为挥发性麻醉剂作用机制的可能性一致。