Yoshida M, Saito H, Katsuki H
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Experientia. 1995 Feb 15;51(2):133-6. doi: 10.1007/BF01929356.
The present study was designed to reveal whether astroglial cells from different brain regions produce diffusible factors that differentially support the survival of neurons and the establishment of neuronal morphology. For this purpose, astrocyte conditioned media (ACM) were prepared by conditioning chemically-defined medium with type I astrocyte culture dissociated from cerebral cortex, hippocampus and hypothalamus. Hippocampal and cortical neurons were cultured in ACM or in non-conditioned medium. ACM derived from three brain regions all supported the survival of hippocampal and cortical neurons better than non-conditioned control medium. Of these, hypothalamic ACM was the most effective in supporting the survival of cortical neurons. The ACM also potentiated the elongation of the longest neurite of hippocampal and cortical neurons. However, there were no significant differences in the promoting effects on neurite elongation among ACM from three brain regions.
本研究旨在揭示来自不同脑区的星形胶质细胞是否产生可扩散因子，这些因子以不同方式支持神经元的存活和神经元形态的建立。为此，通过用从大脑皮层、海马体和下丘脑解离的I型星形胶质细胞培养物处理化学成分确定的培养基来制备星形胶质细胞条件培养基（ACM）。海马体和皮层神经元在ACM或未处理的培养基中培养。来自三个脑区的ACM比未处理的对照培养基更能支持海马体和皮层神经元的存活。其中，下丘脑ACM在支持皮层神经元存活方面最有效。ACM还增强了海马体和皮层神经元最长神经突的伸长。然而，来自三个脑区的ACM对神经突伸长的促进作用没有显著差异。
