Bateson A N, Ultsch A, Darlison M G
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
Gene. 1995 Feb 14;153(2):243-7. doi: 10.1016/0378-1119(94)00776-o.
A genomic clone containing the 5'-flanking sequence of the chicken GABAA receptor alpha 1-subunit-encoding gene (GabR alpha 1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was determined by primer extension, RNase protection and the amplification of chick brain first-strand cDNA. DNA sequence analysis revealed a number of putative transcriptional regulatory motifs, including a TATA box 30 nucleotides upstream from the tsp, and that this region is a CpG island. While there is conservation between the chicken and human GabR alpha 1 sequences, the chicken GabR alpha 1 promoter has a different structure to those reported for the GABAA receptor beta 3- and delta-subunit-encoding genes.
分离并鉴定了一个包含鸡γ-氨基丁酸A型受体α1亚基编码基因（GabRα1）5'侧翼序列的基因组克隆。发现一个内含子打断了5'非翻译区。通过引物延伸、核糖核酸酶保护和鸡脑第一链互补脱氧核糖核酸的扩增确定了转录起始点（tsp）。DNA序列分析揭示了许多假定的转录调控基序，包括在tsp上游30个核苷酸处的一个TATA盒，并且该区域是一个CpG岛。虽然鸡和人GabRα1序列之间存在保守性，但鸡GabRα1启动子的结构与报道的γ-氨基丁酸A型受体β3亚基和δ亚基编码基因的启动子结构不同。
