高效液相色谱法分离和鉴定人血浆中的25-羟基维生素D3 3-硫酸盐

Separation and characterization of 25-hydroxyvitamin D3 3-sulfate in human plasma by high-performance liquid chromatography.

作者信息

Shimada K, Mitamura K, Mukouyama M, Okumura T, Sugaya K

机构信息

Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.

出版信息

J Chromatogr Sci. 1995 Feb;33(2):82-5. doi: 10.1093/chromsci/33.2.82.

DOI:10.1093/chromsci/33.2.82

PMID:7876374

Abstract

The separation and characterization of 25-hydroxyvitamin D3 3-sulfate in human plasma are carried out by high-performance liquid chromatography. The vitamin D sulfate fraction is obtained from a plasma specimen (three volunteers) by the combined use of a Sep-Pak C18 cartridge, for solid-phase extraction, and a lipophilic gel (piperidinohydroxypropyl Sephadex LH-20), for ion-exchange chromatography. 25-Hydroxyvitamin D3 3-sulfate is identified in the examined three specimens in two ways: its chromatographic behavior and that of its fluorescent labeled derivative using 4-[4-(6-methoxy-2-benzoxazolyl)phenyl]-1,2,4-triazoline-3,5- dione; and data obtained from the solvolysis reaction.

摘要

采用高效液相色谱法对人血浆中的25-羟基维生素D3 3-硫酸盐进行分离和表征。维生素D硫酸盐组分是通过联合使用用于固相萃取的Sep-Pak C18柱和用于离子交换色谱的亲脂性凝胶（哌啶基羟丙基葡聚糖凝胶LH-20）从血浆标本（三名志愿者）中获得的。通过两种方式在检测的三个标本中鉴定出25-羟基维生素D3 3-硫酸盐：其色谱行为及其使用4-[4-(6-甲氧基-2-苯并恶唑基)phenyl]-1,2,4-三唑啉-3,5-二酮的荧光标记衍生物的色谱行为；以及从溶剂分解反应获得的数据。