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电子显微镜中颗粒的制备与计数：负染色在琼脂过滤法中的应用

Preparation and counts of particles in electron microscopy: application of negative stain in the agarfiltration method.

作者信息

Kellenberger E, Bitterli D

出版信息

Microsc Acta. 1976 May;78(2):131-48.

PMID:787724
Abstract

The agarfiltration method, published by Kellenberger and Arber in 1957, has been adapted to negative stain with sodium phosphotungstate and uranyl acetate. The technique is described in detail including all recent improvements. The precision obtainable in particle counts is discussed for agarfiltration and sprayed droplets.

摘要

凯伦贝格尔和阿伯于1957年发表的琼脂过滤法已适用于用磷钨酸钠和醋酸铀酰进行负染色。详细描述了该技术，包括所有最新改进。讨论了琼脂过滤和喷雾液滴在颗粒计数中可获得的精度。

相似文献

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Preparation and counts of particles in electron microscopy: application of negative stain in the agarfiltration method.电子显微镜中颗粒的制备与计数：负染色在琼脂过滤法中的应用
Microsc Acta. 1976 May;78(2):131-48.
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Head maturation pathway of bacteriophages T4 and T2. III. Isolation and characterization of particles produced by mutants in gene 17.噬菌体T4和T2的头部成熟途径。III. 基因17突变体产生的颗粒的分离与特性分析。
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