Keiger C J, O'Steen W K, Brewer G, Sorci-Thomas M, Zehnder T J, Rose J C
Department of Physiology and Pharmacology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27157-1083.
Brain Res Mol Brain Res. 1994 Nov;27(1):103-10. doi: 10.1016/0169-328x(94)90190-2.
The regulation of CRF mRNA and protein in the developing ovine brain has been studied to assess the hypothesis that CRF is differentially regulated in the hypothalamus (Hypo), hippocampal-amygdala complex (H & A), frontal cerebral cortex (FCC) and brainstem (BS). We used a quantitative RNase protection assay and radioimmunoassay to determine mRNA and peptide concentrations, respectively, from the last third of gestation until term (i.e., from 95 to 142 days gestation (dg); term approximately 145 days). The major findings from this study are: (1) Hypothalamic CRF mRNA was increased by 2-fold in 140-142 dg fetuses compared to 128-138 and 95-123 dg fetuses; P = 0.016. (2) In the hypothalamus of 140-142 dg fetuses, there was a 2.5-fold increase in CRF mRNA derived from polyadenylation at poly(A) sites 2, 3 or 4; P = 0.005. (3) In 128-138 dg fetuses, CRF mRNA in the frontal cortex was 2-fold higher than in the other brain regions during this time period; P = 0.008. (4) CRF peptide concentrations in the Hypo were 2.5-fold higher in 140-142 dg fetuses compared to 95-106 and 128-138 dg fetuses; P = 0.007. (5) CRF peptide concentrations in the frontal cortex were 5.5-fold higher in 140-142 dg fetuses compared to fetuses at 95-106 dg; P = 0.004. (6) CRF peptide concentrations in the H & A were 5-fold higher in 140-142 dg fetuses compared to 95-106 dg fetuses; P = 0.029. The results from the present study demonstrate for the first time that CRF mRNA and peptide are differentially regulated in a region-specific manner during development.
为了评估促肾上腺皮质激素释放因子（CRF）在下丘脑（Hypo）、海马-杏仁核复合体（H&A）、额叶大脑皮质（FCC）和脑干（BS）中受到差异调节这一假说，研究人员对发育中的绵羊大脑中CRF mRNA和蛋白质的调节进行了研究。我们使用定量核糖核酸酶保护分析和放射免疫分析，分别测定从妊娠最后三分之一期直至足月（即从妊娠95天至142天（dg）；足月约为145天）的mRNA和肽浓度。这项研究的主要发现如下：（1）与妊娠128 - 138天和95 - 123天的胎儿相比，妊娠140 - 142天的胎儿下丘脑CRF mRNA增加了2倍；P = 0.016。（2）在妊娠140 - 142天胎儿的下丘脑中，来自多聚腺苷酸化位点2、3或4的CRF mRNA增加了2.5倍；P = 0.005。（3）在妊娠128 - 138天的胎儿中，在此时间段内额叶皮质中的CRF mRNA比其他脑区高2倍；P = 0.008。（4）与妊娠95 - 106天和128 - 138天的胎儿相比，妊娠140 - 142天的胎儿下丘脑中CRF肽浓度高2.5倍；P = 0.007。（5）与妊娠95 - 106天的胎儿相比，妊娠140 - 142天的胎儿额叶皮质中CRF肽浓度高5.5倍；P = 0.004。（6）与妊娠95 - 106天的胎儿相比，妊娠140 - 142天的胎儿H&A中CRF肽浓度高5倍；P = 0.029。本研究结果首次证明，在发育过程中CRF mRNA和肽以区域特异性方式受到差异调节。
