小脑前体蛋白及一个小脑前体蛋白相关基因的基因组结构与定位

Genomic structure and mapping of precerebellin and a precerebellin-related gene.

作者信息

Kavety B, Jenkins N A, Fletcher C F, Copeland N G, Morgan J I

机构信息

Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.

出版信息

Brain Res Mol Brain Res. 1994 Nov;27(1):152-6. doi: 10.1016/0169-328x(94)90196-1.

DOI:10.1016/0169-328x(94)90196-1

PMID:7877445

Abstract

The cerebellum-specific hexadecapeptide, cerebellin, is derived from a larger precursor, precerebellin, that has sequence homology to the complement component C1qB. We report the cloning of the murine homolog of precerebellin, Cbln1, and a closely related gene, Cbln2. Amino acid comparison of Cbln1 with Cbln2 revealed that Cbln2 is 88% identical to the carboxy terminal region of Cbln1. That these are independent genes was confirmed by Southern analysis and genome mapping. Cbln1 was positioned to the central region of mouse chromosome 8, 2.3 cM distal of JunB and 6.0 cM proximal of Mt1, while Cbln2 mapped to the distal end of mouse chromosome 18, 1.7 cM telomeric of Mbp.

摘要

小脑特异性十六肽小脑素源自一种更大的前体——前小脑素，它与补体成分C1qB具有序列同源性。我们报道了前小脑素的小鼠同源物Cbln1以及一个密切相关基因Cbln2的克隆。Cbln1与Cbln2的氨基酸比较显示，Cbln2与Cbln1的羧基末端区域有88%的同一性。Southern分析和基因组定位证实这些是独立的基因。Cbln1定位于小鼠8号染色体的中央区域，在JunB远端2.3 cM处，在Mt1近端6.0 cM处，而Cbln2定位于小鼠18号染色体的远端，在Mbp的端粒1.7 cM处。