Fairbairn D W, Olive P L, O'Neill K L
Department of Microbiology, Brigham Young University, Provo, UT 84602.
Mutat Res. 1995 Feb;339(1):37-59. doi: 10.1016/0165-1110(94)00013-3.
The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored.
彗星试验是一种用于检测单个细胞中DNA链断裂的灵敏且快速的方法。在过去几年中，其应用显著增加。本文是对迄今为止利用彗星试验发表的研究的综述。讨论了使用该技术的碱性和中性版本进行链断裂检测的原理，并介绍了目前使用的变体的基本方法。综述了该技术在不同领域的应用，并简要探讨了其未来可能的发展方向。
