Greiner C A, Greiner J V, Hebert E, Berthiaume R R, Glonek T
Schepens Eye Research Institute, Boston, MA 02114.
Ophthalmic Res. 1994;26(5):264-74. doi: 10.1159/000267488.
Phospholipids of optic nerve (n = 30) from 5.6-kg rabbits were analyzed by 31P nuclear magnetic resonance (NMR) spectroscopy. Phospholipid metabolites detected were as follows (mol %): phosphatidylcholine (PC; 25.82 +/- 0.12), PC plasmalogen/alkylacyl PC (2.07 +/- 0.13), sphingosylphosphorycholine (1.12 +/- 0.20), phosphatidylinositol (PI; 2.17 +/- 0.21), lyso PC (0.85 +/- 0.06), sphingomyelin (12.52 +/- 0.10); phosphatidylserine (PS; 14.38 +/- 0.11), phosphatidylethanolamine (8.98 +/- 0.11), ethanolamine plasmalogen (28.99 +/- 0.30), unidentified phospholipid (1.10 +/- 0.01), phosphatidic acid (PA; 1.72 +/- 0.06), and lyso PS (0.28 +/- 0.10). The bulk of the ethanolamine phosphatide is in the form of its plasmalogen, which is the major phospholipid detected. The choline plasmalogen, or a reduced derivative thereof, also is present; thus, a significant phospholipid biosynthetic pathway for optic nerve tissue involves the plasmalogen route, which is a pathway distinct from the PA route responsible for the synthesis of PS, PI, and PC. This new 31P NMR lipid analytical technique offers potential for studying optic nerve phospholipid metabolism and degenerating optic nerve tissue, since the technique can accurately quantitate (1) both plasmalogen and nonplasmalogen phospholipids, (2) minor phospholipid components, and (3) previously undetected phospholipids.
采用³¹P核磁共振波谱法对5.6千克家兔的视神经磷脂（n = 30）进行了分析。检测到的磷脂代谢产物如下（摩尔百分比）：磷脂酰胆碱（PC；25.82±0.12）、PC缩醛磷脂/烷基酰基PC（2.07±0.13）、鞘氨醇磷酸胆碱（1.12±0.20）、磷脂酰肌醇（PI；2.17±0.21）、溶血PC（0.85±0.06）、鞘磷脂（12.52±0.10）；磷脂酰丝氨酸（PS；14.38±0.11）、磷脂酰乙醇胺（8.98±0.11）、乙醇胺缩醛磷脂（28.99±0.30）、未鉴定的磷脂（1.10±0.01）、磷脂酸（PA；1.72±0.06）和溶血PS（0.28±0.10）。大部分乙醇胺磷脂以其缩醛磷脂的形式存在，这是检测到的主要磷脂。胆碱缩醛磷脂或其还原衍生物也存在；因此，视神经组织中一条重要的磷脂生物合成途径涉及缩醛磷脂途径，这是一条不同于负责PS、PI和PC合成的PA途径的途径。这种新的³¹P NMR脂质分析技术为研究视神经磷脂代谢和视神经组织退变提供了潜力，因为该技术可以准确地定量（1）缩醛磷脂和非缩醛磷脂，（2）少量磷脂成分，以及（3）以前未检测到的磷脂。
