Wei Y, Ni Z M, Zhou Y, Wu L, Shi L J
Shanghai Institute of Cell Biology, Academia Sinica.
Shi Yan Sheng Wu Xue Bao. 1994 Dec;27(4):505-13.
Among 12 antichromosome antisera from scleroderma patients, four were found to be the antisera against the pellicle of metaphase chromosomes. Western blotting with these sera were achieved on the protein bands resolved by SDS-PAGE of the whole cell lysate and nuclear lysate of the ascites cells. The result showed 11 bands of the nuclear lysate reacted with antisera. Moreover, additional 8 were obtained in the whole cell lysate.
在12份来自硬皮病患者的抗染色体抗血清中，发现有4份是针对中期染色体包膜的抗血清。用这些血清对腹水细胞全细胞裂解物和核裂解物经SDS-PAGE分离后的蛋白条带进行了蛋白质印迹分析。结果显示，核裂解物的11条带与抗血清发生反应。此外，在全细胞裂解物中还得到了另外8条带。
