Chen Y S, Yanagihara N, Murakami S
Department of Otolaryngology, Taiwan Provincial Taipei Hospital, Republic of China.
Acta Otolaryngol. 1994 Nov;114(6):637-42. doi: 10.3109/00016489409126118.
Hypoglossal-facial nerve anastomosis was carried out in 20 adult guinea pigs. Horseradish peroxidase (HRP) was applied to the buccal branch of the facial nerve at intervals of 2, 4 and 6 months after the anastomosis operation. HRP labeled neurons and the number of regenerated axons in the buccal branch were counted to determine postoperative nerve regeneration. The number of surviving neurons in the facial and hypoglossal nuclei was also counted to determine postoperative change of these nuclei. Following anastomosis, 97% of the hypoglossal neurons remained surviving, while the facial neurons underwent pronounced degeneration of 65% survival ratio at 2 months and 37% at 6 months after the anastomosis operation. In 80% of the animals, a new nerve bundle regenerated from the proximal stump of the facial nerve to the anastomotic trunk. A linear increase of HRP-labeled neurons in the facial and hypoglossal nuclei paralleled the increase of the axons in the buccal branch. The HRP-labeled neurons in the facial nucleus were demonstrated to have direct connection with the newly formed bundle and the facial mimetic muscles were dually innervated by both the hypoglossal and facial nerves. Although the present study design might not fully represent the clinical situation, possible advantages of hypoglossal-facial nerve anastomosis are discussed from the view of nerve regeneration.
对20只成年豚鼠进行舌下神经-面神经吻合术。在吻合术后2个月、4个月和6个月时，将辣根过氧化物酶（HRP）应用于面神经颊支。计数HRP标记的神经元以及颊支中再生轴突的数量，以确定术后神经再生情况。还对面神经核和舌下神经核中存活神经元的数量进行计数，以确定这些核的术后变化。吻合术后，97%的舌下神经元存活，而面神经神经元在吻合术后2个月出现明显退变，存活率为65%，6个月时为37%。在80%的动物中，从面神经近端残端到吻合干有新的神经束再生。面神经核和舌下神经核中HRP标记神经元的线性增加与颊支中轴突的增加平行。面神经核中HRP标记的神经元被证明与新形成的神经束有直接连接，面部表情肌由舌下神经和面神经双重支配。尽管本研究设计可能不能完全代表临床情况，但从神经再生的角度讨论了舌下神经-面神经吻合术可能的优势。
