利用海萤荧光素类似物2-甲基-6-（对甲氧基苯基）-3,7-二氢咪唑并[1,2-a]吡嗪-3-酮的化学发光法测定辣根过氧化物酶浓度
Determination of horseradish peroxidase concentration using the chemiluminescence of Cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one.
作者信息
Mitani M, Yokoyama Y, Ichikawa S, Sawada H, Matsumoto T, Fujimori K, Kosugi M
机构信息
Tsukuba Research Laboratory, NOF Corporation, Ibaraki, Japan.
出版信息
J Biolumin Chemilumin. 1994 Nov-Dec;9(6):355-61. doi: 10.1002/bio.1170090602.
The chemiluminescence of the Cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin++ +-3-one (MCLA) was observed at 462 nm in the presence of horseradish peroxidase (HRP) and the total spectrum of light emitted was found to depend linearly on HRP concentration. Methods for the determination of HRP concentration using the chemiluminescence was investigated. HRP could be detected in the range from 100 pmol/L to 100 nmol/L under the optimum condition, H2O2 (10 mmol/L) and MCLA (10 mumol/L) at pH 5.8.
在辣根过氧化物酶（HRP）存在的情况下，在462 nm处观察到海萤荧光素类似物2-甲基-6-（对甲氧基苯基）-3,7-二氢咪唑并[1,2-a]吡嗪-3-酮（MCLA）的化学发光，并且发现发射光的总光谱与HRP浓度呈线性关系。研究了使用化学发光法测定HRP浓度的方法。在最佳条件下，即在pH 5.8时，H2O2（10 mmol/L）和MCLA（10 μmol/L）存在的情况下，HRP的检测范围为100 pmol/L至100 nmol/L。
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