van der Merwe P J, Pieterse J W
Department of Pharmacology, Faculty of Medicine, University of the Orange Free State, Bloemfontein, South Africa.
Analyst. 1994 Dec;119(12):2651-3. doi: 10.1039/an9941902651.
The stability of some growth promoting agents in cattle urine, under various temperature storing conditions, was investigated. Bovine urine samples obtained from previous excretion studies which contained zeranol, nandrolone and trenbolone respectively, were stored frozen, at room temperature and in direct sunlight. On days 0, 2, 4, 7 and 10, zeranol and nandrolone were quantified by selective ion monitoring analysis on a gas chromatography-mass selective detector and trenbolone by using an enzyme linked immunosorbent assay. Zeranol and its metabolite taleranol are stable under all of these storage conditions. There is a remarkable decrease in the concentration of 17 alpha-19-nortestosterone, the main metabolite of nandrolone in cattle, when stored at room temperature and in direct sunlight. There is also a decrease in concentration of trenbolone when stored in direct sunlight. From the results it can be concluded that urine specimens obtained from cattle for residue analysis should be frozen as soon as possible after collection.
研究了某些促生长剂在牛尿液中于不同温度储存条件下的稳定性。从先前排泄研究中分别获取的含玉米赤霉醇、诺龙和群勃龙的牛尿液样本，分别在冷冻、室温及直射阳光下储存。在第0、2、4、7和10天，通过气相色谱 - 质量选择检测器上的选择性离子监测分析对玉米赤霉醇和诺龙进行定量，使用酶联免疫吸附测定法对群勃龙进行定量。玉米赤霉醇及其代谢物塔乐醇在所有这些储存条件下均稳定。当在室温及直射阳光下储存时，牛体内诺龙的主要代谢物17α - 19 - 去甲睾酮的浓度显著降低。在直射阳光下储存时，群勃龙的浓度也会降低。从结果可以得出结论，用于残留分析的牛尿液样本在采集后应尽快冷冻。
